GeneTex

  • Country / Location Selection

United States (US)

SARS-CoV-2 (COVID-19) nsp9 antibody

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135732
Anti-SARS-CoV-2 (COVID-19) nsp9 antibody used in Western Blot (WB). GTX135732
Anti-SARS-CoV-2 (COVID-19) nsp9 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135732

Cat. No. GTX135732

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, PLA, IHC-P (cell pellet)

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) nsp9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP Assay dependent
PLA Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) nsp9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2909871

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135732

GTX135732 ICC/IF Image

SARS-CoV-2 (COVID-19) nsp9 antibody detects SARS-CoV-2 (COVID-19) nsp9 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nsp9 stained by SARS-CoV-2 (COVID-19) nsp9 antibody (GTX135732) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody used in Western Blot (WB). GTX135732

GTX135732 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp9 antibody (GTX135732) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nsp9 antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135732

GTX135732 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nsp9 antibody detects SARS-CoV-2 (COVID-19) nsp9 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) nsp9 transfected 293T.
Green: SARS-CoV-2 (COVID-19) nsp9 stained by SARS-CoV-2 (COVID-19) nsp9 antibody (GTX135732) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Aydin J et al. Nucleic Acids Res 2024; 52 (5) : e26 SHIFTR enables the unbiased identification of proteins bound to specific RNA regions in live cells.
N Schmidt et al. Cell 2023; SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Application : ICC/IF, IP, PLA
Submit a Reference

REVIEW

There are currently no reviews for SARS-CoV-2 (COVID-19) nsp9 antibody (GTX135732). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169